Luteoloside Ameliorates Palmitic Acid-Induced in Vitro Model of Non-alcoholic Fatty Liver Disease via Activating STAT3-Triggered Hepatocyte Regeneration

Luteoloside (Lute), a bioactive natural ingredient, widely exists in nature and possesses hepatoprotective hepatocyte proliferation-promoting properties. This study aimed to investigate whether Lute could counteract non-alcoholic fatty liver disease (NAFLD)-caused damage via its stimulation of regeneration efficacy explore the involved mechanism. LO2 cells primary hepatocytes were used examine proliferation effects under physiological conditions palmitic acid (PA)- induced vitro model NAFLD. STAT3 cell cycle-related proteins (cyclin D1, c-myc p21) evaluated by Western blot. Under conditions, treated with various concentration for 12 24 h showed increased proliferation, especially 20 μM treatment h. More notably, co-incubation also markedly reversed PA-induced inhibition viability hepatocytes. Mechanistically, activate subsequently increase cyclin D1 cmyc expression, which positively regulates cycle progression, decrease expression p21, an inhibitor progression. Furthermore, Luteinduced was abolished stattic. Collectively, can alleviate activating STAT3-mediated regeneration.